| Autor: |
Anran Zheng, Chao Li, Shengkai Xu, Zhen Guo, Chuanyu Li, Changsong Zhang, Jia Yao, Zhiqi Zhang, Jinze Li, Lutao Du, Shasha Zhao, Chuanxin Wang, Wei Zhang, Lianqun Zhou |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
BME Frontiers, Vol 4 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2765-8031 |
| DOI: |
10.34133/bmef.0027 |
| Popis: |
Objective and Impact Statement: We describe an electroenzymatic mediator (EM) sensor based on an electroenzymatic assembly peak separation strategy, which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease (CVD) metabolites in 5 μl of plasma under one test. This work has substantial implications toward improving the efficiency of chronic CVD assessment. Introduction: Monitoring CVD of metabolites is strongly associated with disease risk. Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management. Methods: The EM was flexibly designed by the cross-linking of electron mediators and enzymes, and 3 EM layers with different characteristics were assembled on one electrode. Electrons were transferred under tunable potential; 3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations. Results: In this study, the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM. The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients, and the consistency of risk assessment was 94.6%. Conclusion: Metabolites could be expanded by the EM, and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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