| Autor: |
Muhammad Ajmal Shah, Ruqaiya Khalil, Zaheer Ul-Haq, Pharkphoom Panichayupakaranant |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 36, Iss , Pp 325-331 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2017.07.021 |
| Popis: |
Rhinacanthins-rich extract (RRE) from Rhinacanthus nasutus leaf and its marker compounds namely rhinacanthin-C, rhinacanthin-D and rhinacanthin-N were evaluated for α-glucosidase inhibitory activity. RRE (IC50 value of 25.0 µg/mL) exhibited α-glucosidase inhibitory activity almost equivalent to that of rhinacanthin-C (IC50 value of 22.6 µg/mL) but stronger than that of rhinacanthin-D (IC50 value of 71.5 µg/mL) and the standard drug, acarbose (IC50 value of 395.4 µg/mL), while rhinacanthin-N was inactive. Kinetic studies revealed that both RRE and rhinacanthin-C exhibited noncompetitive α-glucosidase inhibitory activity, while combinations of either RRE or rhinacanthin-C with acarbose at low concentrations (¼IC50, ½IC50 and IC50) showed a synergistic inhibitory effect. In silico studies identified the binding mode of rhinacanthin-C highlighting the formation of both polar and apolar contacts of ligand with α-glucosidase. The present study provides the first evidence that RRE containing rhinacanthin-C as the major compound, could find application as an α-glucosidase inhibitor. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect