| Autor: |
Yichen Guo, Bronte Miller, Michael Heim, Ana Gutierrez-Garcia, Renata Jaskula-Sztul, Bin Ren, Mary Kathryn Sewell-Loftin |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 4, Iss 2, Pp 102177- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2023.102177 |
| Popis: |
Summary: The cross talk between cancer cells and endothelial cells (ECs) within the tumor microenvironment plays a critical role in tumor progression, recurrence, and cancer stemness. Here, we present a protocol containing two in vitro approaches to study such interactions. We first describe an indirect co-culture system to study the regulation of stemness markers in cancer cells by secreted factors from ECs. We then detail a direct co-culture system to study juxtracrine communications between the cell types.For complete details on the use and execution of this protocol, please refer to Sewell-Loftin et al.1 and Guo et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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