| Autor: |
Yifat Eldar-Yedidia, Maskit Bar-Meir, Miriam Hillel, Guila Abitbol, Eti Broide, Roni Falk, Marc Assous, Yechiel Schlesinger |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
PLoS ONE, Vol 11, Iss 2, p e0147883 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0147883 |
| Popis: |
BACKGROUND:Congenital Cytomegalovirus (CMV) is a very common intrauterine infection which can cause severe mental and hearing impairments. Notably, only 40% of primarily infected women transmit CMV to the fetus. CMV-specific T-cell response has a role in CMV disease but individual immune heterogeneity precludes reliable correlation between measurable T-cells response and intrauterine transmission. STUDY AIM:To establish a correlation between maternal T-cells response and fetal CMV transmission using an individual normalized immune response. METHODS:We analyzed IFN-γ secretion upon whole blood stimulation from primary CMV-infected pregnant women, with either CMV-peptides or PHA-mitogen. RESULTS:We established a new normalization method of individual IFN-γ response to CMV by defining the ratio between specific-CMV response and non-specific mitogen response (defined as IFN-γ relative response, RR), aiming to overcome high person-to-person immune variability. We found a unique subpopulation of women with low IFN-γ RR strongly correlated with absence of transmission. IFN-γ RR lower than 1.8% (threshold determined by ROC analysis) reduces the pre-test probability of transmission from 40% to 8%, revealing an unexpected link between low IFN-γ RR and non-transmission. CONCLUSION:In pregnant women with primary CMV infection, low IFN-γ RR is associated with low risk of transmission. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
