Toxicity Assessment of Euphorbia esula L. Extracts on HCT116, SW480, HEK293 Cell Lines, Artemia salina Larvae, and Its Bactericidal Effects
| Autor: | Samaneh Rahamouz-Haghighi, Neda Mohsen-Pour |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of Genetic Resources, Vol 9, Iss 2, Pp 123-132 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2423-4257 2588-2589 |
| DOI: | 10.22080/jgr.2023.24779.1350 |
| Popis: | The aim of this study is to evaluate the potential toxicity of acetonic and methanolic extracts derived from the Euphorbia esula L. plant on various cell lines of human colorectal cancer (HCT116 and SW480), human embryonic kidney normal cells (HEK293), Artemia salina larvae, and its bactericidal effects. The cytotoxic effect of E. esula extracts on cell lines was performed using the MTT assay. In vitro toxicity and biocompatibility of extracts were also evaluated on A. salina and red blood cells by hemolysis test, respectively. The ability of the extracts to inhibit bacterial growth was examined by using the disc diffusion method, as well as the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microtiter broth dilution method. Results showed acetonic extract contains the highest concentration of flavonoid (16.17 µg Qu/mg) and phenol (34.84 µg GA/mg) compared to methanolic extract. The anti-proliferative effects of acetonic extract had the highest effect on HCT116 and HEK293 with IC50 of 64.80 µg/mL and 47.82 µg/mL at 72h, respectively. The hemolysis degree of the methanolic extracts was |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|