New insight into the role of lipid metabolism-related proteins in rheumatic heart valve disease

Autor: Ting Xie, Xuan Chen, Cong Liu, Xingjiu Cai, Mei Xiang, Shiwu Liu, Ruzheng Li, Zhichuan Lin, Debing Liu, Ming Dong, Xinzhong Chen, Minghui Zou, Ping Qiao
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Lipids in Health and Disease, Vol 21, Iss 1, Pp 1-8 (2022)
Druh dokumentu: article
ISSN: 1476-511X
DOI: 10.1186/s12944-022-01722-x
Popis: Abstract Purpose The aim of this study was to determine the expression of lipid metabolism-related proteins in rheumatic heart valve disease (RHVD). Methods This retrospective study involved a total of 20 cases of moderate or severe rheumatic mitral valve stenosis and 4 cases of mitral regurgitation due to secondary causes from September 2018 to September 2021. The patients enrolled included 12 males and 12 females who underwent surgical excision of the mitral valve at the cardiac surgery department of Hainan General Hospital. The samples of mitral valve were collected during surgery treatment as the study group, and mitral valves collected from patients with ischemic heart disease were allocated into the control group. Hematoxylin–eosin (HE), oil red staining and immunohistochemical (IHC) staining were conducted to compare the expression of lipid metabolism-related proteins (ATP-binding cassette transporter A1 and acyl-coenzyme A: cholesterol acyltransferase-1), and real-time polymerase chain reaction (RT–PCR) was applied to compare the mRNA levels of ABCA1, ACAT1, and the inflammatory cytokines TNF-α, IL-10, and MCP-1. Results In general, the rheumatic mitral valve showed leaflet thickening along with border adhesions and visible yellow fats. Oil red O staining also revealed the abovementioned results as well as fat cells. Both ABCA1 and ACAT1 were expressed in the rheumatic mitral valve via IHC, whereas only ACAT1 showed a faint level of expression in the ischemic mitral valve with no expression of ABCA1. In addition, compared with the ischemic mitral valve, RT-PCT showed increased mRNA expression levels of ABCA1, ACAT1, and the inflammatory cytokines TNF-α, IL-10, and MCP-1 (P
Databáze: Directory of Open Access Journals
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