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Abstract Effective management of rare species requires an understanding of spatial variation in abundance, which is challenging to estimate. We tested the efficacy of high‐resolution imagery to detect burrows of the Texas kangaroo rat (TKR; Dipodomys elator) as a means of estimating abundance across its geographic range. Specifically, we estimated burrow counts using an Unmanned Aerial System (UAS) to collect data from very high‐resolution Red–Green–Blue (RGB) imagery and estimate digital elevation (2.5‐mm pixel resolution) over active and inactive burrows located on mesquite mounds and anthropogenic features (roadsides, fences, etc.). In 2018, we identified 26 burrow locations on a private ranch in Wichita County, Texas, USA, and characterized burrows based on topography and vegetation density. We found that TKR burrows can only be identified with data of |