Analysis of Seven Performance Evaluation Program Results for Pathogenic Bacteria from Laboratory Animals in 2013—2020
| Autor: | XING Jin, FENG Yufang, WANG Hong, ZHANG Xueqing, GAO Qiang, YUE Bingfei, FU Rui |
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| Jazyk: | čínština |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Shiyan dongwu yu bijiao yixue, Vol 41, Iss 6, Pp 521-527 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1674-5817 88996980 |
| DOI: | 10.12300/j.issn.1674-5817.2021.104 |
| Popis: | ObjectiveTo promote the development of domestic laboratory animal detection proficiency testing activities and testing levels by assessing self testing capabilities.MethodsThe National Institutes for Food and Drug Control participated in the International Council for Laboratory Animal Science (ICLAS) laboratory animal detection proficiency test (i.e. Performance Evaluation Program for Diagnostic Laboratories, PEP) in 2013—2020. PEP pathogenic bacteria samples were tested by culture, biochemical identification, PCR, and sequencing. Finally, the results were analyzed and summarized.ResultsThere were 40 samples in seven international PEP activities, involving 27 kinds of pathogenic bacteria. The coincidence rates of the seven results were 8/8, 2/2, 5/8, 9/9, 5/5, 6/7, and 1/1, respectively, including 33 pathogen detection items and 7 antibody detection items. Nine pathogens were missing from the national standards: Bordetella hinzii, Serratia marcescens, Corynebacterium mastitis, CAR (Cilia-associated respiratory) bacillus, Actinobacillus muris, Citrobacter freundii, Klebsiella oxytoca, Streptococcus agalactiae, and pathogenic Escherichia coli.ConclusionThrough participating in PEP, we can identify the differences between domestic and foreign standards in laboratory animal detection and the testing capabilities that we need to improve, expecting to be in line with international standards as soon as possible. |
| Databáze: | Directory of Open Access Journals |
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