Glycerol Enhancement of Ligand-Polylysine/DNA Transfection
| Autor: | W. Zauner, A. Kichler, W. Schmidt, A. Sinski, E. Wagner |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 20, Iss 5, Pp 905-913 (1996) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/96205rr04 |
| Popis: | Primary human fibroblasts and a series of cell lines (A549, BNL CL.2, H225, NIH 3T3 and Rat-1) are efficiently transfected by using positively charged complexes of plasmid DNA and transferrin-polylysine or polylysine in the presence of glycerol (1 molar to 1.8 molar, depending on the cell type). An increase in gene expression of up to several- hundredfold (compared to complexes without glycerol) is obtained if the transfection mixture is incubated with the cells for 3–4 h at 37°C. This simple method has been used for transient expression of luciferase, b-galactosidase and interleukin-2, and also for the generation of stably transfected cells. |
| Databáze: | Directory of Open Access Journals |
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