Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing
| Autor: | Ling Zhang, Xiang-dong Yang, Yuan-yu Zhang, Jing Yang, Guang-xun Qi, Dong-quan Guo, Guo-jie Xing, Yao Yao, Wen-jing Xu, Hai-yun Li, Qi-yun Li, Ying-shan Dong |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | International Journal of Genomics, Vol 2014 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2314-436X 2314-4378 |
| DOI: | 10.1155/2014/921950 |
| Popis: | The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybean GmFad2-1b 801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoter BCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression of GmFad2-1b in the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to |
| Databáze: | Directory of Open Access Journals |
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