Cell–Cell Mating Interactions: Overview and Potential of Single-Cell Force Spectroscopy

Autor:	Peter N. Lipke, Jason M. Rauceo, Albertus Viljoen
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	atomic force microscopy cell–cell mating adhesion single-cell force spectroscopy yeasts bacteria Biology (General) QH301-705.5 Chemistry QD1-999
Zdroj:	International Journal of Molecular Sciences, Vol 23, Iss 3, p 1110 (2022)
Druh dokumentu:	article
ISSN:	1422-0067 1661-6596
DOI:	10.3390/ijms23031110
Popis:	It is an understatement that mating and DNA transfer are key events for living organisms. Among the traits needed to facilitate mating, cell adhesion between gametes is a universal requirement. Thus, there should be specific properties for the adhesion proteins involved in mating. Biochemical and biophysical studies have revealed structural information about mating adhesins, as well as their specificities and affinities, leading to some ideas about these specialized adhesion proteins. Recently, single-cell force spectroscopy (SCFS) has added important findings. In SCFS, mating cells are brought into contact in an atomic force microscope (AFM), and the adhesive forces are monitored through the course of mating. The results have shown some remarkable characteristics of mating adhesins and add knowledge about the design and evolution of mating adhesins.
Databáze:	Directory of Open Access Journals
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