| Popis: |
Azadirachtin is a rapidly developing botanical pesticide with toxicity to arthropods. GST is a multifunctional biological enzyme that can remove peroxide and promote drug metabolism. In this study, RT-PCR technology was used to clone the GSTM3, GSTT1 and GSTD1 genes of Eriocheir hepuensis. The results showed that the coding sequence (CDS) lengths of the GSTM3, GSTT1 and GSTD1 genes were 675 bp, 687 bp and 666 bp, encoding 224, 228 and 221 amino acids, respectively. The deduced protein domain prediction showed that the GSTM3, GSTT1 and GSTD1 proteins of Eriocheir hepuensis had the GST-N-Mu and GST-C-family, GST-N-T and GST-C-T, and GST-N-D and GST-C-D domains, respectively. In normal tissues, the expression levels of GSTM3, GSTT1 and GSTD1 were highest in the hepatopancreas. Under azadirachtin stress, compared with the control group, the GSTM3, GSTT1 and GSTD1 genes showed a double peak (both at 6 h and 24 h) in the hepatopancreas and a single peak (peak of GSTD1 in gills at 9 h, others at 6 h) in the heart and gills, while the expression of GSTM3, GSTT1 and GSTD1 in the dead individuals in the experimental group was lower than that in the surviving individuals. These results showed that individual death under azadirachtin stress is related to its ability to regulate the expression of GSTM3, GSTT1 and GSTD1, and the three GST genes played an important role in Eriocheir hepuensis regulating azadirachtin stress. |
