Lactobacillus paracasei probiotic properties and survivability under stress-induced by processing and storage of ice cream bar or ice-lolly

Autor: André Fioravante Guerra, Wilson José Fernandes Lemos Junior, Geraldo Oliveira dos Santos, Christian Andrighetto, Alessio Gianomini, Viviana Corich, Rosa Helena Luchese
Jazyk: English<br />Portuguese
Rok vydání: 2018
Předmět:
Zdroj: Ciência Rural, Vol 48, Iss 9 (2018)
Druh dokumentu: article
ISSN: 1678-4596
0103-8478
DOI: 10.1590/0103-8478cr20170601
Popis: ABSTRACT: Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. The aim of this study was to identify genotypically lactobacilli strains isolated from newborn stools and selecting strain based on probiotic properties (gastrointestinal tolerance, antibiotic susceptibility, inhibition of pathogen biofilm formation, absence of alfa or gamma-blood hemolysis, and lysozyme sensibility) and technological properties of surviving either in ice cream bar or ice-lolly. Reduction of 1.2log cfu ml-1 of the Lactobacillus paracasei strain was observed after exposure through in vitro gastrointestinal conditions. It inhibited biofilms of Escherichia coli, Salmonella Typhimurium and Candida albicans by mechanisms of competition, exclusion and displacement, and was resistant up to 3000μg ml-1 of egg white lysozyme. It presented neither alfa nor gamma-hemolysis or was antibiotic resistant to usual antibiotics for human use. Microbial survivability in ice cream bar or ice-lolly was assessed up to 21 days of storage at -18°C. Viability was maintained in ice cream bar, but there was a reduction of almost 2.0logs in ice-lolly.
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