Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration

Autor: Dong Joon Lee, Patricia Miguez, Jane Kwon, Renie Daniel, Ricardo Padilla, Samuel Min, Rahim Zalal, Ching-Chang Ko, Hae Won Shin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Tissue Engineering, Vol 11 (2020)
Druh dokumentu: article
ISSN: 2041-7314
20417314
DOI: 10.1177/2041731420981672
Popis: Scaffolds that are used for bone repair should provide an adequate environment for biomineralization by mesenchymal stem cells (MSCs). Recently, decellularized pulp matrices (DPM) have been utilized in endodontics for their high regenerative potential. Inspired by the dystrophic calcification on the pulp matrix known as pulp stone, we developed acellular pulp bioscaffolds and examined their potential in facilitating MSCs mineralization for bone defect repair. Pulp was decellularized, then retention of its structural integrity was confirmed by histological, mechanical, and biochemical evaluations. MSCs were seeded and proliferation, osteogenic gene expression, and biomineralization were assessed to verify DPM’s osteogenic effects in vitro. MicroCT, energy-dispersive X-ray (EDX), and histological analyses were used to confirm that DPM seeded with MSCs result in greater mineralization on rat critical-sized defects than that without MSCs. Overall, our study proves DPM’s potential to serve as a scaffolding material for MSC-mediated bone regeneration for future craniofacial bone tissue engineering.
Databáze: Directory of Open Access Journals
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