| Autor: |
G. -X. Tang, K. Knecht, X. -F. Yang, Y. -B. Qin, W. -J. Zhou, D. Cai |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Biologia Plantarum, Vol 55, Iss 1, Pp 21-26 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1573-8264 |
| DOI: |
10.1007/s10535-011-0003-0 |
| Popis: |
A two-step protocol for improving the frequency of shoot regeneration from oilseed rape (Brassica napus L.) hypocotyl explants was established. The protocol consists of a pre-culture on callus induction medium (CIM) and a subsequent shoot regeneration on shoot induction medium (SIM). The SIM was Murashige and Skoog medium supplemented with different concentrations of 6-benzylaminopurine (BA; 2-5 mg dm-3) and naphthaleneacetic acid (NAA; 0.05-0.15 mg dm-3). Maximum frequency of shoot regeneration (13 %) was on the SIM medium containing 4 mg dm-3 BA and 0.1 mg dm-3 NAA, but it increased to 24.45 % when 20 μM silver thiosulphate (STS) was added. Strikingly, an extremely high frequency of shoot regeneration up to 96.67 % was reached by a two-step protocol when hypocotyl explants had been pre-cultured for 7 d on a CIM medium containing 1.5 mg dm-3 2,4-dichlorophenoxyacetic acid. In addition, the shoot emergence was also 7 d earlier than that observed by use of the one-step protocol. The two-step protocol was also applied for regeneration of transgenic plants with cZR-3, a nematode resistance candidate gene. As a result, 43 plants were generated from 270 shoots and from these 6 plants proved to be transgenic. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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