miR-132-3p downregulates in CD4 T cells and is associated with disease manifestations in patients with lupus
| Autor: | Haihong Qin, Sunyi Chen, Xiao Liu, Jun Liang, Hao Wu, Xiaohua Zhu |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Journal of International Medical Research, Vol 52 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1473-2300 03000605 |
| DOI: | 10.1177/03000605241286762 |
| Popis: | Objective This study aimed to evaluate the expression status of miR-132-3p in CD4 + T cells in patients with systemic lupus erythematosus (SLE) and explore its potential role in SLE development. Methods The study included 60 patients with SLE and 30 healthy controls. miR-132-3p expression in CD4 + T cells was detected by real-time quantitative reverse transcription polymerase chain. Bioinformatics analyses were employed to predict target genes and explore the potential role of miR-132-3p. The associations between miR-132-3p levels and SLE Disease Activity Index (SLEDAI) score, as well as laboratory characteristics, were analyzed. Results miR-132-3p levels in CD4 + T cells were significantly higher in patients with SLE compared with healthy controls. Bioinformatics analysis identified FOXO1 as a potential target gene of miR-132-3p, with a particular emphasis on the FOXO signaling pathway. miR-132-3p up-regulation in CD4 + T cells was associated with high SLEDAI score, high anti-double-stranded DNA levels, low C3 and C4 levels, positive anti-ribosomal P, and high 24-hour urinary protein levels in patients with SLE. Conclusions miR-132-3p may contribute to CD4 + T cell dysregulation during SLE by targeting FOXO1 and could potentially be used to assess disease severity. |
| Databáze: | Directory of Open Access Journals |
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