Maintenance of Francisella tularensis 15 RIEH and Brucella abortus 19 BA Strains in a Viable State by Means of Deep Freezing
| Autor: | A. N. Malakhaeva, O. Yu. Lyashova, O. P. Plotnikov, A. V. Osin |
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| Jazyk: | ruština |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Проблемы особо опасных инфекций, Vol 0, Iss 1, Pp 63-66 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0370-1069 2658-719X |
| DOI: | 10.21055/0370-1069-2015-1-63-66 |
| Popis: | Assessed are the methods for storage of microorganisms in a viable state under various technological conditions for a period of two years. Compared is the survivability of the test-strains of tularemia and brucellosis agents (exemplified by Francisella tularensis 15 RIEH and Brucella abortus 19 BA) when stored on the nutrient media at temperatures raging 0 °C up +8 °C and at -70 °C for over a year. Made has been an estimate of survivability of the vaccine strains stored at -70 °C within two years term. It is determined that optimum media for storing the stated above microorganisms with no changes in their morphological characteristics at -70 °C are sucrose-gelatin agar and Albimi broth with 10 % glycerin. Demonstrated is the fact that it is more efficient to conserve microorganisms stored in working collections by way of deep freezing. |
| Databáze: | Directory of Open Access Journals |
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