CRISPR/Cas9 application in cancer therapy: a pioneering genome editing tool

Autor: Sadegh Shojaei Baghini, Zhanna R. Gardanova, Saeme Azizi Hassan Abadi, Burhan Abdullah Zaman, Ahmet İlhan, Navid Shomali, Ali Adili, Roozbeh Moghaddar, Amirhossein Fakhre Yaseri
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Cellular & Molecular Biology Letters, Vol 27, Iss 1, Pp 1-37 (2022)
Druh dokumentu: article
ISSN: 1425-8153
1689-1392
DOI: 10.1186/s11658-022-00336-6
Popis: Abstract The progress of genetic engineering in the 1970s brought about a paradigm shift in genome editing technology. The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system is a flexible means to target and modify particular DNA sequences in the genome. Several applications of CRISPR/Cas9 are presently being studied in cancer biology and oncology to provide vigorous site-specific gene editing to enhance its biological and clinical uses. CRISPR's flexibility and ease of use have enabled the prompt achievement of almost any preferred alteration with greater efficiency and lower cost than preceding modalities. Also, CRISPR/Cas9 technology has recently been applied to improve the safety and efficacy of chimeric antigen receptor (CAR)-T cell therapies and defeat tumor cell resistance to conventional treatments such as chemotherapy and radiotherapy. The current review summarizes the application of CRISPR/Cas9 in cancer therapy. We also discuss the present obstacles and contemplate future possibilities in this context.
Databáze: Directory of Open Access Journals
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