| Autor: |
Kamyshny A.M. |
| Jazyk: |
Russian<br />Ukrainian |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
Морфологія, Vol 3, Iss 1, Pp 38-43 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1997-9665 |
| Popis: |
Peculiarities of protein р53 expression in rat thymus with experimental diabetes mellitus were investigated and discussed in experimental work. Investigations were carried out on 28 male rats Vistar line 5-6 months of age. Experimental diabetes mellitus was designed by intraperitoneal injection of streptozotocyn (SIGMA, USA) at dose 50 mg/kg one time. Method of double immunofluorescence with usage of rat monoclonal anti-p53 and anti-СD4 antibodies was used for revelation of p53. Structure of a thymus was analyzed with the help of software VIDAS. Control animals showed an increase of p53+-cells quantity in thymus cortical zone by 58% as compared with medullar zone. Even so immature forms of p53+-cells – big lym-phocytes and p53+-lymphoblasts prevailed in population of p53+-cells of both thymus cortical zone and medullar zone. It has been established that development of experimental diabetes mellitus was accompanied by increase in number of p53+-cells and concentration of protein р53 in thymus cortical zone in the absence of any changes in medullar zone. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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