Immunohistological changes in skin wounds during the early periods of healing in a rat model
| Autor: | F. Sabol, L. Dancakova, P. Gal, T. Vasilenko, M. Novotny, K. Smetana, L. Lenhardt |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Veterinární Medicína, Vol 57, Iss 2, Pp 77-82 (2012) |
| Druh dokumentu: | article |
| ISSN: | 0375-8427 1805-9392 |
| DOI: | 10.17221/5253-VETMED |
| Popis: | The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin 14, α-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo. |
| Databáze: | Directory of Open Access Journals |
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