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Objective: Observing the effects of polysaccharide from red kidney beans (PRK) combined with exercise (E) to improve the diet induced obese mice metabolic disorders, and analyzing its mechanism. Methods: C57BL/6 mice were randomly divided into 5 groups: Control group (normal diet), model group (high fat diet), PRK group (400 mg/kg PRK), E group (exercise), PRK+E group (400 mg/kg PRK+exercise). After continuous intervention for 12 weeks, oral glucose tolerance test (OGTT) was performed, and blood glucose, insulin, total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) were measured by biochemical analyzer. TC, TG, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were analyzed in liver. The contents of insulin, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β were determined by enzyme-linked immunoassay kit. The levels of PPARα, FASN, Nrf2, NQO1 and HO-1 were detected by western blot. Results: Compared with the control group, the body weight and liver weight of model group were significantly increased (P |
