Autor: |
Koen J. A. Martens, Sam P. B. van Beljouw, Simon van der Els, Jochem N. A. Vink, Sander Baas, George A. Vogelaar, Stan J. J. Brouns, Peter van Baarlen, Michiel Kleerebezem, Johannes Hohlbein |
Jazyk: |
angličtina |
Rok vydání: |
2019 |
Předmět: |
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Zdroj: |
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019) |
Druh dokumentu: |
article |
ISSN: |
2041-1723 |
DOI: |
10.1038/s41467-019-11514-0 |
Popis: |
Single-particle tracking PALM (sptPALM) provides quantitative information in vivo if the protein of interest remains in a single diffusional state during track acquisition. Here the authors develop a custom-built sptPALM microscope and a Monte-Carlo based diffusion distribution analysis to study dynamic DNA-dCas9 interactions in live bacteria. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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