Visualisation of dCas9 target search in vivo using an open-microscopy framework

Autor: Koen J. A. Martens, Sam P. B. van Beljouw, Simon van der Els, Jochem N. A. Vink, Sander Baas, George A. Vogelaar, Stan J. J. Brouns, Peter van Baarlen, Michiel Kleerebezem, Johannes Hohlbein
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-019-11514-0
Popis: Single-particle tracking PALM (sptPALM) provides quantitative information in vivo if the protein of interest remains in a single diffusional state during track acquisition. Here the authors develop a custom-built sptPALM microscope and a Monte-Carlo based diffusion distribution analysis to study dynamic DNA-dCas9 interactions in live bacteria.
Databáze: Directory of Open Access Journals