Ficus elastica Leaf Extract Effect Toward IL-10 and TNF-α as a Preeclampsia Model on Hypoxia-induced EA.hy926
| Autor: | Chrismis Novalinda Ginting, I Nyoman Ehrich Lister, Ermi Girsang, Maya Sari Mutia, Yolanda Eliza Putri Lubis, Annisa Amalia, Wahyu Setia Widodo, Dwi Surya Artie, Rizal Rizal, Wahyu Widowati |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Journal of Mathematical and Fundamental Sciences, Vol 53, Iss 3 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2337-5760 2338-5510 |
| DOI: | 10.5614/j.math.fund.sci.2021.53.3.10 |
| Popis: | Preeclampsia is a pregnancy complication characterized by proteinuria, hypertension, hypoxia as well an increased systemic inflammatory response. It affects 5 to 10% of all births and is a leading factor in fetal and maternal morbidity and death. Ficus elastica L. leaves are known to have natural compounds with anti-inflammatory and antioxidant properties. This research assessed the potential of F. elastica leaf extract (FEE) as anti-inflammatory agent in a hypoxic-induced human endothelial cell line (EA.hy926) as preeclampsia model. F. elastica leaves were extracted using 70% ethanol. Cytotoxic assay of the FEE in different concentrations (3.13 to 100 µg/ml) against EA.hy926 was done using MTS assay. The FEE’s anti-inflammatory activity was measured with ELISA for the TNF-α and IL-10 parameters. The FEE did not cause cytotoxicity to the EA.hy926 cells, with viability above 80% at the highest concentration. The FEE could decrease the pro-inflammatory TNF-α level and increase the IL-10 level as anti-inflammatory markers in the preeclampsia model at significance level p |
| Databáze: | Directory of Open Access Journals |
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