| Autor: |
H.L. Brockman, F.J. Kézdy, J.H. Law |
| Jazyk: |
angličtina |
| Rok vydání: |
1975 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 16, Iss 1, Pp 67-74 (1975) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)36752-3 |
| Popis: |
The rate of lipolytic enzyme-catalyzed reactions yielding water-soluble products can be measured by isobaric titration. The method is based upon the measurement of the amount of substrate that must be added to a monalayer to maintain constant surface pressure during the course of the enzymatic reaction. The rate constants determined for the hydrolysis of trioctanion and 1,2-diotanoin by pancreatic lipase were identical with those determined by the variable surface pressure method and by a radioactive substrate technique. This direct titrimetric method has a wider dynamic range and more versatility for following surface reactions that previously described systems. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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