Novel approach for accurate tissue-based protein colocalization and proximity microscopy

Autor:	Mirjam I. Lutz, Carmen Schwaiger, Bernhard Hochreiter, Gabor G. Kovacs, Johannes A. Schmid
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	Medicine Science
Zdroj:	Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Druh dokumentu:	article
ISSN:	2045-2322
DOI:	10.1038/s41598-017-02735-8
Popis:	Abstract Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could demonstrate that distinct neurodegeneration-related proteins either not or only rarely interact in human brain tissue.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/2af7528ddb9b4f7ebaf9ee2b229fe643 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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