MicroRNA profiling of ovarian granulosa cell tumours reveals novel diagnostic and prognostic markers
| Autor: | Wei-Tzu Cheng, Roseanne Rosario, Anita Muthukaruppan, Michelle K Wilson, Kathryn Payne, Peter C. Fong, Andrew N. Shelling, Cherie Blenkiron |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Clinical Epigenetics, Vol 9, Iss 1, Pp 1-10 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1868-7075 1868-7083 |
| DOI: | 10.1186/s13148-017-0372-0 |
| Popis: | Abstract Background The aim of this study was to explore the clinical utility of microRNAs (miRNAs) as improved markers of ovarian granulosa cell tumours (GCTs) for cancer diagnosis and prognosis prediction. Current histopathological and genetic markers, such as the presence of a FOXL2 gene mutation to distinguish between the two major subtypes are not wholly accurate and as such novel biomarkers are warranted. Methods The miRNA expression profiles of five formalin-fixed, paraffin-embedded (FFPE) adult-GCTs and five juvenile-GCTs were assessed using Affymetrix miRNA 3.0 Arrays and compared for differential expression. Ten miRNAs were assessed in an additional 33 FFPE tumours and four normal granulosa cell samples by quantitative RT-PCR, and their expression correlated to clinical information. Results MicroRNA array found 37 miRNAs as differentially expressed between the two GCT subtypes (p |
| Databáze: | Directory of Open Access Journals |
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