A novel fluorogenic probe for visualizing the hydrogen peroxide in Parkinson’s disease models

Autor: Gaobin Zhang, Zheng Li, Fangjie Chen, Duoteng Zhang, Wenhui Ji, Zhengpeng Yang, Qiong Wu, Chengwu Zhang, Lin Li, Wei Huang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Innovative Optical Health Sciences, Vol 13, Iss 3, Pp 2050013-1-2050013-10 (2020)
Druh dokumentu: article
ISSN: 1793-5458
1793-7205
17935458
DOI: 10.1142/S1793545820500133
Popis: Parkinson’s disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H2O2) in organisms. Developing an efficient method for noninvasive and real-time H2O2 detection is beneficial to investigate the role played by H2O2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H2O2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H2O2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H2O2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H2O2 detection, it has been successfully applied to visualizing the H2O2 concentration in living cells, Zebrafish and C. elegans PD models.
Databáze: Directory of Open Access Journals
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