Pharmacokinetics of Ginkgolide B after Oral Administration of Three Different Ginkgolide B Formulations in Beagle Dogs
| Autor: | Jie Zhao, Ting Geng, Qi Wang, Haihong Si, Xiaoping Sun, Qingming Guo, Yanjing Li, Wenzhe Huang, Gang Ding, Wei Xiao |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Molecules, Vol 20, Iss 11, Pp 20031-20041 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1420-3049 20111967 |
| DOI: | 10.3390/molecules201119678 |
| Popis: | Ginkgolide B (GB), an important active constituent of Ginkgo biloba extract, has been used in clinical applications for the treatment of dementia, cerebral insufficiency or related cognitive decline. To investigate the main pharmacokinetic characteristics of three different GB formulations in beagle dogs, a simple, specific and sensitive LC-MS/MS method was established and validated. The separation of the analytes was achieved on an Agilent Eclipse Plus C18 column (1.8 μm, 2.1 × 50 mm) with a mobile phase consisting of water and acetonitrile. The flow rate was set at 0.4 mL/min. Quantitation was performed using multiple reaction monitoring (MRM) in negative ion mode, with the transitions at m/z (Q1/Q3) 423.1/367.1 for GB and m/z 269.3/170.0 for IS. The linear calibration curve of GB was obtained over the concentration range of 2–200 ng/mL. The intra- and inter-day precisions were |
| Databáze: | Directory of Open Access Journals |
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