| Autor: |
G.Q. Rodrigues, I.M.T. Lima, R.N. Chaves, R. Rossetto, S.L. Costa, S.V. Castro, V.R.P. Barros, M.H.T. Matos, C.A.P. Lopes, S.N. Báo, C.C. Campello, J.R. Figueiredo |
| Jazyk: |
English<br />Portuguese |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 66, Iss 2, Pp 411-416 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1678-4162 |
| DOI: |
10.1590/1678-41626023 |
| Popis: |
The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral follicles regardless of its concentration, however, larger follicles diameter (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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