| Autor: |
Abdullah Ünlü, Ömür Baysal, İlknur Polat, Serap Melike Sülü, Hatice İkten, Zübeyir Devran, Emine Gümrükçü |
| Jazyk: |
English<br />Turkish |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Derim, Vol 34, Iss 2, Pp 122-130 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1300-3496 |
| DOI: |
10.16882/derim.2017.349991 |
| Popis: |
The aim of this study was to diagnos bacterial speck of tomato (Pseudomonas syringae pv. tomato) pathogen using biochemical and molecular methods and to determine genetical diversity of Pst isolates by using ISSR and SRAP molecular markers. In the study, survey studies were carried out in West Mediterranean Region to collect bacterial pathogen of tomato (Pst). 10 Pst isolates were collected during survey studies. After isolation, the pathogen was diagnosed with biochemical and molecular diagnostic methods. Besides, genetic diversities of Pst isolates were determined using ISSR and SRAP molecular markers. As a result, bacterial speck of tomato was seen depending on the climatic conditions at the region and Pst was successfully detected by using classical and molecular methods. ISSR and SRAP markers were successively used for analyzing genetic diversity of Pst isolates and these primers could be efficiently used to separate isolates of disease agent. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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