In vivo detection of optically-evoked opioid peptide release

Autor: Ream Al-Hasani, Jenny-Marie T Wong, Omar S Mabrouk, Jordan G McCall, Gavin P Schmitz, Kirsten A Porter-Stransky, Brandon J Aragona, Robert T Kennedy, Michael R Bruchas
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: eLife, Vol 7 (2018)
Druh dokumentu: article
ISSN: 2050-084X
DOI: 10.7554/eLife.36520
Popis: Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.
Databáze: Directory of Open Access Journals