| Popis: |
The Mango parasite is a parasitic plant that is very detrimental to mango plant owners but has the potential as a producer of secondary metabolites which are expected to inhibit the growth of the fungus Candida albicans. This study aimed to obtain antifungal activity fractions, to obtain pure compound eluates which were active as antifungals, to determine the class of compounds active as antifungals, and to determine the Minimum Inhibitory Concentration (MIC) against the fungus C. albicans. Based on the research that has been done, the fractions that are active as antifungals are the n-hexane and ethyl acetate fractions. The results of purification of the n-hexane and ethyl acetate fractions using gravity column chromatography showed the presence of two pure n-hexane compounds that were active in inhibiting C. albicans, namely N-1 and N4 eluates with inhibition zone diameters of 12 mm and 10 mm, respectively. Purification of the ethyl acetate fraction produced two active compounds, namely eluate E-1 and eluate E-5 which were able to inhibit C. albicans by 10 mm and 12 mm, respectively. The MIC values for N-1, N-4, and E-1 eluates were able to inhibit at a concentration of 500 ppm and were classified as quite strong. The E-5 eluate capable of inhibiting at a concentration of 1000 ppm is classified as weak. Tests for the class of compounds using Thin Layer Chromatography (TLC) and the R¦ value of the biautography results showed the color spots on the N-1 eluate (R¦: 0.9) were blue in the terpenoid group, on the N-4 eluate (R¦: 0.6) Alkaloid group is orange in color, the E-1 eluate (R¦: 0.8) is yellow in the Phenolic group, the E-5 eluate (R¦: 0.9) is purple in the terpenoid group. |
