| Autor: |
Wencan LI, Qichang XING, Xiang LIU, Wei LI, Zheng LIU, Jiani ZHANG |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Holistic Integrative Pharmacy, Vol 3, Iss 4, Pp 388-395 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2707-3688 |
| DOI: |
10.1016/S2707-3688(23)00007-9 |
| Popis: |
Objective: To observe the protective effect of Buyang Huanwu decoction (BYHWD) against retinal inflammation in diabetic rats. Methods: Healthy male Wistar rats were randomly divided into normal and diabetic model groups. Diabetic rats were given a one-time intraperitoneal injection of streptozotocin (STZ) to establish a diabetic phenotype and were randomly assigned to the negative control (saline), positive control (calcium dobesilate), and BYHWD treatment groups. After 8 weeks of treatment, fluorescein-labeled dextran and Evans blue dye were injected into the tail vein to observe vascular permeability and vascular leakage in the retina, respectively. Perfusion of fluorescein-labeled concanavalin was used to detect adhesion in rat retinal leukocytes. Western blotting was used to quantify the expression levels of the inflammatory factors TNF-α, NF-κBp65, and p-NF-κBp65 in retinal tissue. Results: Compared with nondiabetic rats, saline-treated diabetic rats showed significantly increased retinal vascular leakage, leukocyte adhesion, and protein levels of TNF-α and p-NF-κBp65 (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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