Extravisual opsins in the blind shrimp Creaseria morleyi: presence and expression

Autor: José R. Pérez-Calderón, Jorge A. Pérez-León, Nuno Simões, Marisela Aguirre-Ramírez, Roxana E. Malpica-Calderón, Alejandro Botello
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Nauplius, Vol 31 (2023)
Druh dokumentu: article
ISSN: 2358-2936
DOI: 10.1590/2358-2936e2023024
Popis: Abstract The presence of a long-wavelength sensitive (LWS) opsin gene was demonstrated in the stygobitic crustacean Creaseria morleyi (Creaser, 1936) by PCR readings from genomic DNA. In order to find the expression of this gene in extraocular tissue, shrimps were collected and placed in a tank to expose them to light/dark conditions for a period of 72 hours, and immediately after, sacrificed and sampled for RNA in the eyes, cephalothorax, abdomen, and sixth abdominal segment plus uropods. The transcripts of the LWS opsin gene were found in the eyes and abdomen of individuals exposed to light. The expression of these opsins could be involved in extravisual functions such as synchronization of their biological processes with environmental cycles related to diurnal vertical migration.
Databáze: Directory of Open Access Journals