| Autor: |
Rhodin C. Joseph, Matheus Silva da Fonseca Diniz, Viviane Magno do Nascimento, Abraão de Jesus Barbosa Muribeca, Johan Carlos Costa Santiago, Luziane da Cunha Borges, Paulo Roberto da Costa Sá, Paulo Wender Portal Gomes, Júlio César da Silva Cardoso, Marcela Natalia Rocha de Castro, Thais Fiusa, Hervé Rogez, Sylvain Darnet, Mara Silvia Pinheiro Arruda, Milton Nascimento da Silva, Alberto Cardoso Arruda, Jean A. Boutin, Consuelo Yumiko Yoshioka e Silva, Emmanuelle Lautié |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Molecules, Vol 25, Iss 24, p 5992 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1420-3049 |
| DOI: |
10.3390/molecules25245992 |
| Popis: |
The main challenge of plant chemical diversity exploration is how to develop tools to study exhaustively plant tissues. Their sustainable sourcing is a limitation as bioguided strategies and dereplication need quite large amounts of plant material. We examine if alternative solutions could overcome these difficulties by obtaining a secure, sustainable, and scalable source of tissues able to biosynthesize an array of metabolites. As this approach would be as independent of the botanical origin as possible, we chose eight plant species from different families. We applied a four steps culture establishment procedure, monitoring targeted compounds through mass spectrometry-based analytical methods. We also characterized the capacities of leaf explants in culture to produce diverse secondary metabolites. In vitro cultures were successfully established for six species with leaf explants still producing a diversity of compounds after the culture establishment procedure. Furthermore, explants from leaves of axenic plantlets were also analyzed. The detection of marker compounds was confirmed after six days in culture for all tested species. Our results show that the first stage of this approach aiming at easing exploration of plant chemodiversity was completed, and leaf tissues could offer an interesting alternative providing a constant source of natural compounds. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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