Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (Oreochromis spp.)

Autor: Moisés Villanueva, Guillermo Espinosa-Reyes, Rogelio Flores-Ramirez, Angel Natanael Rojas-Velazquez, Juan Carlos García López, Anayeli Vazquez-Valladolid, José Alejandro Roque-Jimenez, German D. Mendoza-Martinez, Pedro A. Hernandez-Garcia, Monika Palacios-Martinez, Alfonso J. Chay-Canul, Héctor A. Lee-Rangel
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Life, Vol 12, Iss 8, p 1243 (2022)
Druh dokumentu: article
ISSN: 2075-1729
DOI: 10.3390/life12081243
Popis: This experiment aimed to evaluate the effects of herbal vitamin C at different levels on tilapia (Oreochromis spp.) growth, potential DNA damage, and the metabolomic profile of water effluent. Forty-five tilapias were housed in separate plastic tanks (80 L), and these were randomly assigned to three treatments: (a) a commercial diet (CD) only; (Nutripec Purina®); (b) the commercial diet plus 250 mg of herbal vitamin C (HVC)/kg (CD250); and (c) the commercial diet plus 500 mg of HVC/kg (CD500). Biometric measurements were taken each week, blood samples were collected from the caudal vein on the final day, and water effluent was taken each week and immediately frozen (−80 °C) until further analysis (gas chromatography/mass spectrometry (GC/MS) systems). Data were completely randomized with a 2 × 2 factorial arrangement of treatments. Upon including herbal vitamin C, the final BW (p = 0.05) and BWG (p = 0.06) increased linearly. Herbal vitamin C decreases DNA damage (p ≥ 0.05). PLS-DA showed a 41.6% variation between treatments in the water samples. Fifteen metabolites had the best association between treatments, with a stronger correlation with CD500. Herbal vitamin C could improve fish performance, prevent DNA damage, and influence changes in the metabolomic profile of the water.
Databáze: Directory of Open Access Journals
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