Apolipoprotein H: a two-step isolation method

Autor: R Gambino, G Ruiu, M Cassader, G Pagano
Jazyk: angličtina
Rok vydání: 1996
Předmět:
Zdroj: Journal of Lipid Research, Vol 37, Iss 4, Pp 902-904 (1996)
Druh dokumentu: article
ISSN: 0022-2275
DOI: 10.1016/S0022-2275(20)37588-X
Popis: A new method for the purification of apolipoprotein H by affinity chromatography followed by continuous-elution electrophoresis is described. It is both simpler and less complicated than the chromatographic and electrophoretic methods usually used. In addition, apolipoprotein H is isolated in a pure, structurally uncleaved form. This is of importance, as impairment has been detected in commercial preparations. The separation and purification of apolipoprotein H is a necessary prelude to its quantitative determination and phenotyping, and hence the clarification of its physiopathological mechanisms in lipid metabolism.
Databáze: Directory of Open Access Journals