HLA‐DR3介导的CD4 T细胞对1型糖尿病患者GAD65的应答

Autor: Neihenuo Chuzho, Neetu Mishra, Nikhil Tandon, Uma Kanga, Gunja Mishra, Akanksha Sharma, Narinder K. Mehra, Neeraj Kumar
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Diabetes, Vol 15, Iss 7, Pp 607-621 (2023)
Druh dokumentu: article
ISSN: 1753-0407
1753-0393
DOI: 10.1111/1753-0407.13406
Popis: Abstract Aim We planned this study to identify diabetogenic glutamic acid decarboxylase (GAD65) peptides possibly responsible for human leucocyte antigen (HLA)‐DR3/DQ2‐mediated activation of GAD65‐specific CD4 T cells in type 1 diabetes (T1D). Methods Top 30 GAD65 peptides, found to strongly bind in silico with HLA‐DR3/DQ2 molecules, were selected and grouped into four pools. The peptides were used to stimulate CD4 T cells of study subjects in 16‐h peripheral blood mononuclear cell culture. CD4 T cells' stimulation in terms of interferon‐gamma (IFN‐γ), interleukin (IL)‐17, tumor necrosis factor‐alpha (TNF‐α), and IL‐10 expression was analyzed using flow cytometry. Results Although all four GAD65 peptide pools (PP1‐4) resulted in significantly higher expression of IFN‐γ by CD4 T cells (p = .003, p
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