PREVENTION METHODS AGAINST Aeromonas hydrophila and Pseudomonas fluorescens INFECTION IN TILAPIA

Autor: Esti Handayani Hardi, Rudi Agung Nugroho, Irawan Wijaya Kusuma, Wiwin Suwinarti, Ryan Anjani, Aziza Aziza
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Biotropia: The Southeast Asian Journal of Tropical Biology, Vol 27, Iss 3 (2020)
Druh dokumentu: article
ISSN: 0215-6334
1907-770X
DOI: 10.11598/btb.0.0.0.1131
Popis: This research evaluated a method involving provision of a concoction of Boesenbergia pandurata, Solanum ferox dan Zingimber zerumbet extracts for pathogen prevention in tilapia. The concentration of each extract was 600 ppm of Boesenbergia pandurata/BP, 900 ppm of Solanum ferox/SF and 200 ppm of Zingimber zerumbet/ZZ. The examination was performed by issuing two combinations of extracts (SF:BP, SF:ZZ) against Aeromonas hydrophila and Pseudomonas fluorescens (105 CFUmL-1). Preventive trials were carried out by providing a concoction of extracts through intraperitoneal injection (0.1 mL/fish) in tilapia (15±2 g) and the immersion method was performed by bathing the fish in the extracts for 20 minutes, with pathogen challenging during the following 24 h being carried out. The composition of the used extract was by SF60:ZZ40; SF50:ZZ50; BP90:SF10; BP50:SF50; and fish without being given the extract. Haematology and immunology parameters were observed at the 4th week after challanges with pathogenic bacteria. The number of white blood cells (WBCs) increased significantly (P
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