| Autor: |
Sebastian Eggert, Melanie Kahl, Nathalie Bock, Christoph Meinert, Oliver Friedrich, Dietmar W. Hutmacher |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Materials & Design, Vol 204, Iss , Pp 109619- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
0264-1275 |
| DOI: |
10.1016/j.matdes.2021.109619 |
| Popis: |
Gelatin methacryloyl (GelMA)-based hydrogels have become highly studied as modular tissue culture platforms due to the combination of the bioactivity of gelatin and tailorability of photo-crosslinkable hydrogels. However, current production and characterization workflows still rely on manual, time-consuming, and low-throughput processes, ultimately limiting widespread adaption. To address these challenges, an open-source technology platform is engineered and validated for automated high-throughput production of GelMA-based 3D constructs by integrating automated pipetting capabilities for viscous and non-viscous liquids. Firstly, the platform is applied in combination with inexpensive absorbance measurements to systematically optimize the mixing sequence for GelMA-based precursor solutions. This enables a decrease in the relative standard deviation of the prepared mixtures from 63% to 2.5%, demonstrating a significantly improved reproducibility. Next, the applicability and high-throughput capacity are demonstrated by the reproducible generation of GelMA dilution series with 0.5 and 2% (w/v) concentration steps as well as double network hydrogels consisting of 2% (w/v) alginate and 5% (w/v) GelMA. Finally, a simple and user-friendly methodology is described that integrates Design of Experiments approaches to systematically study the combinatorial effects of material and crosslinking parameters and to establish a parameter library for on-demand production of mechanically defined GelMA-based hydrogels. The platform enables reproducible production and offers an open-source and accessible alternative to high-priced liquid-handling robots and bioprinters. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect