Probiotic microorganisms affect the reproductive and nervous systems of male rats treated with acrylamide
| Autor: | Seyedhossein Hekmatimoghaddam, Maryam Yadegari, Fateme Zare, Fatemeh Zakizadeh, Seyed Mohammad Seifati |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Asian Pacific Journal of Reproduction, Vol 13, Iss 4, Pp 178-186 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2305-0500 2305-0519 |
| DOI: | 10.4103/apjr.apjr_33_24 |
| Popis: | Objective: To evaluate the protective effects of probiotic microorganisms on the reproductive and nervous systems of male rats treated with acrylamide. Methods: Thirty-two rats were randomly divided into 4 groups and received normal saline through gavage (control), acrylamide 20 mg/kg body weight, acrylamide plus probiotic microorganisms (Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Bifidobacterium breve, Bifidobacterium infantis, Streptococcus thermophilus and fructooligosaccharides, all mixed in sachets) 20 or 200 mg/kg body weight, respectively. After 30 days, the testis, prostate, seminal vesicle and cerebellum were removed, fixed and stained with hematoxylin-eosin (H & E). The Johnsen score was used to classify spermatogenesis. Cavalieri's principle method was used to evaluate the total volume (in mm3) of the testes. The number of each intratubular cell type as well as intertubular Leydig cells in whole samples was measured using the physical dissector counting techniques. Stereological analysis and the grids were used to determine the volume of cerebellar layers as well as the Purkinje cell number. Results: The testis weight decreased significantly in the acrylamide- treated group compared to the other groups (P |
| Databáze: | Directory of Open Access Journals |
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