IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells
| Autor: | Roni M. Shtein, Susan G. Elner, Zong-Mei Bian, Victor M. Elner |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | International Journal of Inflammation, Vol 2012 (2012) |
| Druh dokumentu: | article |
| ISSN: | 2090-8040 2042-0099 |
| DOI: | 10.1155/2012/714704 |
| Popis: | Purpose. To determine time course of effect of lipopolysaccharide (LPS) on production of interleukin-8 (IL-8) and monocyte chemotactic protein (MCP) by cultured human corneal stromal cells. Methods. Human corneal stromal cells were harvested from donor corneal specimens, and fourth to sixth passaged cells were used. Cell cultures were stimulated with LPS for 2, 4, 8, and 24 hours. Northern blot analysis of IL-8 and MCP gene expression and ELISA for IL-8 and MCP secretion were performed. ELISA results were analyzed for statistical significance using two-tailed Student's t-test. Results. Northern blot analysis demonstrated significantly increased IL-8 and MCP gene expression after 4 and 8 hours of exposure to LPS. ELISA for secreted IL-8 and MCP demonstrated statistically significant increases (P |
| Databáze: | Directory of Open Access Journals |
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