| Autor: |
Lei Zhang, Xian-Jin Zhou, Li-Ying Zhan, Xiao-Jing Wu, Wen-Lan Li, Bo Zhao, Qing-Tao Meng, Zhong-Yuan Xia |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Brazilian Journal of Anesthesiology, Vol 67, Iss 6, Pp 600-606 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
0104-0014 |
| DOI: |
10.1016/j.bjane.2017.02.002 |
| Popis: |
Background and objectives: Dexmedetomidine (DEX) has demonstrated the preconditioning effect and shown protective effects against organize injury. In this study, using A549 (human alveolar epithelial cell) cell lines, we investigated whether DEX preconditioning protected against acute lung injury (ALI) in vitro. Methods: A549 were randomly divided into four groups (n = 5): control group, DEX group, lipopolysaccharides (LPS) group, and D-LPS (DEX + LPS) group. Phosphate buffer saline (PBS) or DEX were administered. After 2 h preconditioning, the medium was refreshed and the cells were challenged with LPS for 24 h on the LPS and D-LPS group. Then the malondialdehyde (MDA), superoxide dismutase (SOD), Bcl-2, Bax, caspase-3 and the cytochrome c in the A549 were tested. The apoptosis was also evaluated in the cells. Results: Compare with LPS group, DEX preconditioning reduced the apoptosis (26.43% ± 1.05% vs. 33.58% ± 1.16%, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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