利用UPLC-DAD-HRMS分析低温压榨 鲜青花椒油贮藏期间的色素变化Analysis of pigment change of cold-pressed fresh green Huajiao (Zanthoxylum schinifolium Sieb. et Zucc) oil by UPLC-DAD-HRMS

Autor: 刘若曦1,2,肖宇雪1,2,俸贵强1,2,章康致1,2,周纯洁3,蔡甜4,5,陈科伟1,2,5,6,7 LIU Ruoxi1,2, XIAO Yuxue1,2, FENG Guiqiang1,2, ZHANG Kangzhi1,2, ZHOU Chunjie3, CAI Tian4,5, CHEN Kewei1,2,5,6
Jazyk: English<br />Chinese
Rok vydání: 2024
Předmět:
Zdroj: Zhongguo youzhi, Vol 49, Iss 6, Pp 145-152 (2024)
Druh dokumentu: article
ISSN: 1003-7969
DOI: 10.19902/j.cnki.zgyz.1003-7969.230592
Popis: 旨在为低温压榨鲜青花椒油的工业生产、贮藏和质量控制提供借鉴,利用超高效液相色谱-二极管阵列检测器-高分辨质谱(UPLC-DAD-HRMS)技术对低温压榨鲜青花椒油中色素进行测定,并研究贮藏过程中光、氧、温度对低温压榨鲜青花椒油的色素含量和色泽L*、a*、b*的影响。结果表明:从低温压榨鲜青花椒油中鉴定出32种叶绿素和10种类胡萝卜素;叶绿素中主要含有叶绿素a、C132-羟基-叶绿素a、叶绿素b和脱镁叶绿素a;类胡萝卜素中主要含有叶黄素和β-胡萝卜素;叶绿素总量为9.44 mg/kg,类胡萝卜素总量为4.95 mg/kg。贮藏过程中影响低温压榨鲜青花椒油中色素降解最主要的因素是光,其次是温度,氧气几乎没有影响。另外,a*可以作为衡量低温压榨鲜青花椒油色素含量及色泽控制的指标。Aiming to contribute to the industrial production, storage and quality control of cold-pressed fresh green Huajiao oil, the change of pigment in cold-pressed fresh green Huajiao oil was analyzed by ultra-high performance liquid chromatography-diode array detector-high resolution mass spectrometry (UPLC-DAD-HRMS), and the effects of light, oxygen, temperature on the pigment content and color(L*, a*, b*) of fresh green Huajiao oil in storage were determined. The results showed that 32 chlorophylls and 10 carotenoids were identified from the cold-pressed fresh green Huajiao oil. The main chlorophylls were chlorophyll a, C132-hydroxy chlorophyll a, chlorophyll b and pheophytin a; the main carotenoids were lutein and β-carotene. The total chlorophyll content was 9.44 mg/kg, and the total carotenoid content was 4.95 mg/kg. During storage, the main factor affecting the degradation of pigment in fresh green Huajiao oil was light, followed by temperature, and oxygen had little effect. In addition, the color index a* can be used as an index for pigment content and color control.
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