RAPD-based genetic relationships in different Bougainvillea cultivars

Autor: Richa Srivastava, Sudhir Shukla, Arvind Soni, Anil Kumar
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Crop Breeding and Applied Biotechnology, Vol 9, Iss 2, Pp 154-163 (2009)
Druh dokumentu: article
ISSN: 1518-7853
1984-7033
Popis: The present study deals with authenticating existing knowledge about 21 Bougainvillea cultivars comprisingof 9 hybrids and their parents through RAPD analysis. The 19 degenerate primer sets generated 234 bands from which 158(67.5%) were polymorphic. The UPGMA based dendrogram divided 21 cultivars into two major groups with Jaccard’ssimilarity coefficient ranging from 0.51 to 0.942. Group A had three cultivars namely Trinidad, Formosa and Dr. H. B. Singhin which Dr. H.B. Singh was confirmed as a hybrid of other two cultivars. Group B was sub divided into 8 clusters. Theparentages of 7 out of 8 hybirds have been confirmed based on clusters. The study concluded that the RAPD technique issuitable for confirmation of parent-hybrid relationship.
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