Distribution of nesfatin-1 expression in Bactrian camels and its impact on adipocyte glucose metabolism
| Autor: | Siriguleng Yu, Bijun Chen, Haoyu Bai, Ziyi Li, Yaru Niu, Xingchuan He, Wen Yu, Shumin Du, Junjian Jin, Hongqiang Yao |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Pesquisa Veterinária Brasileira, Vol 44 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1678-5150 28544129 |
| DOI: | 10.1590/1678-5150-pvb-7464 |
| Popis: | ABSTRACT: The study aimed to investigate the distribution and expression of nesfatin-1 in Bactrian camels and its impact on adipocyte glucose metabolism. Polyclonal antibodies against a single antigenic epitope of NUCB2/nesfatin-1 protein were prepared. Nesfatin-1 expression was detected in various adipose tissues, including the hypothalamus, humps, stomach, duodenum, jejunum, ileum, cecum, colon, rectum, pancreas, liver, and abdomen, using Western blot and qRT-PCR. Glucose uptake levels, pyruvic acid content, and hexokinase and phosphofructokinase activities were measured in cultured 3T3-L1 preadipocytes treated with nesfatin-1 in a high glucose state using a non-radiofluorimetric assay. The results showed that nesfatin-1 was found in all Bactrian camel tissues, with higher adipose tissue and pancreas expression. However, qRT-PCR analysis revealed higher expression of nesfatin-1 mRNA in the abdominal fat and liver. After nesfatin-1 treatment, a significant decrease (p |
| Databáze: | Directory of Open Access Journals |
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