Intensiometric biosensors visualize the activity of multiple small GTPases in vivo

Autor: Jihoon Kim, Sangkyu Lee, Kanghoon Jung, Won Chan Oh, Nury Kim, Seungkyu Son, YoungJu Jo, Hyung-Bae Kwon, Won Do Heo
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-018-08217-3
Popis: FRET sensors hardly achieve visualization of spatiotemporal dynamics of protein activity in vivo. Here the authors present intensiometric small GTPase biosensors based on dimerization-dependent fluorescent proteins that enable monitoring of activity of small GTPases in the brains of behaving mice at a single spine resolution.
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