Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

Autor: Laura Toribio, Irene Magdaleno, Beatriz Martín-Gómez, María T. Martín, Silvia Valverde, Ana M. Ares
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Separations, Vol 10, Iss 1, p 9 (2022)
Druh dokumentu: article
ISSN: 10010009
2297-8739
DOI: 10.3390/separations10010009
Popis: The enantiomeric separation of antifungal compounds is an arduous task in pharmaceutical and biomedical fields due to the different properties that each diastereoisomer presents. The enantioseparation of a group of fungicides (sulconazole, bifonazole, triadimefon and triadimenol) using supercritical fluid chromatography was achieved in this work. For this goal, four different chiral columns based on polysaccharide derivatives, as well as the effect of different chromatographic parameters such as temperature, type and percentage of organic modifier (methanol, ethanol and isopropanol), were thoroughly investigated. The inversion of the elution order of enantiomers as a result of a change in the stationary phase or organic modifier was also evaluated by employing a circular dichroism detector. The best separation conditions, in terms of the enantioresolution and analysis time, were obtained with the Lux® Cellulose-2 column using isopropanol as the organic modifier.
Databáze: Directory of Open Access Journals
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