Effects of Cigarette Smoke and Opium on the Expression of CD9, CD36, and CD68 at mRNA and Protein Levels in Human Macrophage Cell Line THP-1
| Autor: | Mohammad Amin Momeni-Moghaddam, Gholamreza Asadikaram, Mohammad Hadi Nematollahi, Mojdeh Esmaeili Tarzi, Sanaz Faramarz-Gaznagh, Abbas Mohammadpour-Gharehbagh, Mohammad Kazemi Arababadi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Iranian Journal of Allergy, Asthma and Immunology, Vol 19, Iss 1 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1735-1502 1735-5249 |
| DOI: | 10.18502/ijaai.v19i1.2417 |
| Popis: | Cigarette smoking and opium use are risk factors for coronary artery disease (CAD). It has been known that scavenger receptors such as CD36 and CD68 play critical roles in the pathogenesis of CAD. CD9, as a member of the tetraspanin, has been shown to interact with scavenger receptors. The aim of this study was to investigate the effects of these risk factors on expression levels of CD9, CD36, and CD68 on the THP-1 cell line. The THP-1 cell line treated with cigarette smoke extract (CSE( and opium, both individually and combinatory, in 24 h incubation. The protein and mRNA levels of CD9, CD36, and CD68 were evaluated by flow cytometry and quantitative reverse transcription-Polymerase Chain Reaction (qRT-PCR) techniques, respectively. CD36 and CD68 mRNA and protein expression levels were significantly increased in the cells treated with cigarette smoke extract compared to the control (p |
| Databáze: | Directory of Open Access Journals |
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